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Confocal images of MEFs 30 h after treatment with indicated concentrations of ActD for apoptosis induction. DMSO was applied as control (i.e., 0 nM ActD). 30 mins before apoptosis induction, cells were either pre-treated with M1 (“5 µM M1”) or vehicle (DMSO, “0 µM M1”). A-V: Annexin V; scale bars: 100 µm.
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Confocal images of MEFs 30 h after treatment with indicated concentrations of ActD for apoptosis induction. DMSO was applied as control (i.e., 0 nM ActD). 30 mins before apoptosis induction, cells were either pre-treated with M1 (“5 µM M1”) or vehicle (DMSO, “0 µM M1”). A-V: Annexin V; scale bars: 100 µm.

Journal: Scientific Data

Article Title: Mitochondrial apoptosis imaged by high-throughput confocal microscopy

doi: 10.1038/s41597-026-08502-7

Figure Lengend Snippet: Confocal images of MEFs 30 h after treatment with indicated concentrations of ActD for apoptosis induction. DMSO was applied as control (i.e., 0 nM ActD). 30 mins before apoptosis induction, cells were either pre-treated with M1 (“5 µM M1”) or vehicle (DMSO, “0 µM M1”). A-V: Annexin V; scale bars: 100 µm.

Article Snippet: For apoptosis induction in MEFs, cells were seeded in 384-well μClear imaging plates (Greiner, 781091) at a density of 24,000 cells/cm and media was changed 24 hours later.

Techniques: Confocal, Control